Selective media for pseudomonas pseudomonas sigmaaldrich. Pseudomonas aeruginosa produces a number of water soluble iron chelators, including the yellowgreen or yellowbrown fluorescent pyoverdin. A new medium for the detection of fluorescent pigment production by. Pseudomonas aeruginosa forms fluorescent green colonies while the growth of klebsiella, proteus, and providencia is strongly suppressed enhanced pigment production aids identification. Thirtynine strains of 10 different species of pseudomonas. Pseudomonas can, in rare circumstances, cause communityacquired pneumonias, as well as ventilatorassociated pneumonias, being one of the most common agents isolated in several studies. Pseudomonas isolation agar is not intended for use in the diagnosis of disease or other conditions in humans pseudomonas isolation agar is based on medium a described by king, ward, and raney. Thermo scientific remel pseudomonas f agar flo agar is used for the differentiation of pseudomonas aeruginosa from other pseudomonas species based on fluorescein production. Pseudomonas aeruginosa is a common encapsulated, gramnegative, rodshaped bacterium that can cause disease in plants and animals, including humans.
If growth is observed on pseudomonas f agar, fluorescein production is determined by visual examination of the. A nonselective or selective agar plate may be used for inoculation of the test isolate. Select the center of a wellisolated colony and streak onto pseudomonas f agar. Pseudomonas aeruginosa produces a variety of watersoluble pigments. Pseudomonas isolation agar base, used for the selective isolation and identification of p. Pseudomonas agar base is designed so that by the addition of the appropriate supplement sr0102 or sr0103 the medium becomes selective for pseudomonas aeruginosa or pseudomonas spp. Cetrimide pseudosel agar is used for the selective isolation and. This medium is very useful for isolating pseudomonas spp.
Pseudomonas is a genus of gramnegative, gammaproteobacteria, belonging to the family pseudomonadaceae and containing 191 validly described species. Using the oxidase test and cetrimide agar to identify pseudomonas. A new selective medium for isolating pseudomonas spp. Pseudomonas putida will be incubated in a 150 ml of lb broth at 37 celsius with shaking 100 rpm. Pseudomonas selective agar base untuk mikrobiologi granucult find msds or sds, a coa, data sheets and more information. The ratio of peptones in peptospecial is conducive of fluorescein production by pseudomonas. As the name suggests, it contains cetrimide, which is the selective agent against alternate microbial flora. I want to select a suitable time period for the incubation until bacterial growth reached the. Using the oxidase test and cetrimide agar to identify. The base medium is a modification of kings a medium 1 in which magnesium chloride and potassium sulphate are present to enhance pigment production. Pseudomonas isolation agar also includes glycerol as an energy source, while peptone provides nutrients necessary for bacterial growth. Cetrimide also enhances the production of pseudomonas pigments such as pyocyanin and pyoverdine, which show a.
Cetrimide agar is used as a selective medium for the isolation of pseudomonas aeruginosa from pus, sputum and drains, etc. Pseudomonas aeruginosa pseudomonas aeruginosa is a gramnegative, rodshaped, motile organism polar flagella which characteristically produce watersoluble pigments which diffuse through the medium. Glycerol serves as an energy source and also helps to promote pyocyanin production. Pseudomonas agar base is a modification of kings a medium 5 which contains magnesium chloride and potassium sulphate to enhance pigment production. Pseudomonas isolation agar includes irgasan, a potent broadspectrum antimicrobial not active against pseudomonas spp. Pseudomonas agar p is mentioned in the fda bacteriological analytical. Pseudomonas aeruginosa colonies on tryptic soy agar. Cetrimide agar composition, principle, uses, preparation. Summary pseudomonas aeruginosa is an environmental isolate commonly found in soil, water, food, and in many manmade products. Technical information pseudomonas agar medium for detection of fluorescein product code. Technical information pseudomonas agar medium for detection. Pseudomonas cn selective agar when supplemented with pseudomonas cn selective supplement 1. Pseudomonas agar p base for microbiology find msds or sds, a coa, data sheets and more information.
Iso 720 and iso 16266 granucult find msds or sds, a coa, data sheets and more information. Section 1 identification of the substancemixture and of the. Pseudomonas isolation agar is used for the isolation of pseudomonas aeruginosa and other pseudomonas spp. Pseudomonas isolation agar is prepared according to a slight modification of the medium a formulation of king, ward and raney. Pseudomonas aeruginosa morphology, growth characteristics and. Cultivation 24 hours in an aerobic atmosphere, 37c. Pseudomonas aeruginosa is the most common and important species colonising the lungs of cystic fibrosis patients contributing to an accelerated decline in lung function. Recommended for selective isolation of pseudomonas species. Pseudomonas isolation agar base m406 composition ingredients gms litre peptone 20. The organism can utilize an array of nutrients and will thrive in a variety of environments, including medical equipment such as catheters, infusion fluids, disinfectants, and cosmetics. Storage requirements jun 12, 2018 cetrimide agar composition, principle, uses, preparation and colony morphology last updated. Pseudomonas f agar is used to detect pigment production by pseudomonas species. Food and drug administration 10903 new hampshire avenue silver spring, md 20993 1888infofda 18884636332 contact fda.
It consists of 350 micrograms of nitrofurantoin per ml and 2 micrograms of crystal violet per ml in a nutrient agar base. An opportunistic, nosocomial pathogen of immunocompromised individuals, p. Antibiotic susceptibility test of biofilm producing bacteria was performed by using the kirbybauer disc diffusion technique. Pseudomonas can, in rare circumstances, cause communityacquired pneumonias, as well as ventilatorassociated pneumonias, being one of the most common. Student streaks on pseudomonas isolation agar as observed following lab 3. Some pseudomonas strains elaborate both pigments, while others elaborate only one of the two. Cled agar supports the growth of all potential urinary pathogens, and a number of contaminants such as diphtheroids, lactobacilli, and micrococci.
Pseudomononas agar base is a modification of kings a medium 1 which contains magnesium chloride and potassium sulphate to enhance pigment production. A novel chromogenic medium for isolation of pseudomonas. The best known are pyocyanin bluegreen, pyoverdine yellowgreen, fluorescent, and pyorubin redbrown, produced by a small proportion of strains. Section 1 identification of the substancemixture and of. Pseudomonas cn selective agar ready to use plates 55 and 90 mm. Pseudomonas produce a variety of pigments, and fluorescein is commonly produced by pseudomonas aeruginosa. This video lesson demonstrates how to identify a bacterium as pseudomonas aeruginosa by using the oxidase test and selective cetrimide agar.
Typical composition gliter peptone from gelatine 16. A new medium, pseudomonas selectiveisolation agar, was. Pancreatic digest of casein 10gl, proteose peptone no. Pseudomonas agar base for microbiology sigmaaldrich. Pseudomonas agar medium for detection of fluorescein is used for the detection of fluorescein production by pseudomonas species, and is in accordance with united states pharmacopoeia 2008. Incubate the plate in ambient air caps loosened for slants at. Gelatin and meat peptones provide the nutrients necessary for bacterial growth. A new medium, pseudomonas selective isolation agar, was developed to isolate pseudomonas spp. Pseudosel agar or cetrimide agar is used for the selective isolation and presumptive identification of pseudomonas aeruginosa. Experimental procedure and evaluation pseudomonas cfc selective agar. Colonisation rates are estimated at 80% in adults and 60% in the cf population overall. Their ease of culture in vitro and availability of an increasing number of pseudomonas strain. The medium contains pigmentenhancing components and the selective agents, triclosan 4 which selectively inhibits nonpseudomonads. The optimal composition of the medium per litre was bacto peptone 10 g, gel.
Incubate the plate in ambient air caps loosened for slants at 3337c for 1824 hours. Section 1 identification of the substancemixture and of the company 1. Pseudomonas p agar is used to isolate, and detect pigment production by pseudomonas aeruginosa. Add several drops of chloroform to the growth and observe for a bluegreen color. Can nutrient agarbroth be used for pseudomonas aeruginosa in.
Cetrimide agar is a type of agar used for the selective isolation of the gramnegative bacterium, pseudomonas aeruginosa. Pyocyanin is a blue, phenazine pigment that diffuses into the agar medium to give the surrounding medium a blue coloration. Cfc pseudomonas agar, base, cn pseudomonas agar, base, cetrimide nalidixic acid agar, base, pp pseudomonas agar, base. It is the most common cause of infections of burn injuries and of the external ear otitis.
Cfc pseudomonas agar, base cn pseudomonas agar, base cetrimide nalidixic acid agar, base pp pseudomonas agar, base pseudomonas agar base may be used for the selective detection of pseudomonas aeruginosa pathogens in mineral water sample that are mainly responsible for food and waterborne infections. Form of product poured plate storage 6 12c filling weight 17 g 5 % packaging 10 plates wrapped in film ph 7. Pseudomonas p agar, resulting in a variety of hues from blue to red. Pseudomonas aeruginosa morphology, growth characteristics. Pseudomonas isolation agar medium for pseudomonas aeruginosa. Pseudomonas isolation agar is based on medium a described by king, ward, and raney. If growth is observed on pseudomonas f agar, fluorescein. The medium is modification of kings a medium 1 and contains magnesium chloride and potassium sulphate for improved pigment production and recovery of pseudomonas aeruginosa while strongly suppressing klebsiella, proteus and providencia spp. Selectively isolate pseudomonas species from a range of samples with thermo scientific oxoid pseudomonas agar base dehydrated. Staphylococcus aureus and pseudomonas aeruginosa biofilm. Pseudomonas colonies into the medium on which they grow. Can nutrient agarbroth be used for pseudomonas aeruginosa. Streak from the liquid enrichment on a solid medium of the same composition, as observed following lab 2.
Pseudomonas agar f and p tech agar hardy diagnostics. Cetrimide agar is recommended in the united states pharmacopoeia xxvi 1 and european pharmacopoeia iv 2 for use in microbial limit tests. Page 1 of 7 sds 9222 rev 0 november 2015 section 1 identification of the substancemixture and of the company 1. Goto and enomoto 2 formulated cetrinix supplement for the selective isolation of pseudomonas aeruginosa from. If pigment production is questionable small amount of bluegreen color confirm the reaction by extraction with chloroform. The test isolate should be in pure culture and 1824 hours old. Irgasan is incorporated into the medium to inhibit the growth of many grampositive and gramnegative microorganisms other than pseudomonas spp. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Macconkey agar contains the dye crystal violet well as. Pseudomonas aeruginosa growing on macconkey agar macconkey agar is a selective medium used for the isolation of nonfastidious gramnegative rods, particularly members of the family enterobacteriaceae and the genus pseudomonas, and the differentiation of lactose fermenting from lactose nonfermenting gramnegative bacilli. Cetrimide also enhances the production of pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic bluegreen and yellowgreen colour. Pseudomonas aeruginosa is the most common cause of infections of burn injuries and of the outer ear otitis externa, and is the most frequent colonizer of medical devices e.
The medium was first developed by lowburry and is a modification of tech agar developed by king et al. June 12, 2018 by sagar aryal cetrimide agar is a selective and differential medium used for the isolation and identification of pseudomonas aeruginosa from clinical and nonclinical specimens. Jul 05, 2016 this video lesson demonstrates how to identify a bacterium as pseudomonas aeruginosa by using the oxidase test and selective cetrimide agar. Pseudomonas agar p, also known as tech agar, is recommended for enhancement of pyocyanin production by pseudomonas aeruginosa. The base medium is a modification of kings medium a which uses magnesium and potassium salts to enhance production of the pigments pyocyanin green and fluorescein detected by u. Cetrimide agar is a selective and differential medium used for the isolation and identification of pseudomonas aeruginosa from clinical and nonclinical specimens cetrimide is the selective agent and inhibits most bacteria by acting as a detergent cetyltrimethylammonium bromide, a quaternary ammonium, cationic detergent. Pseudosel agar or cetrimide agar is used for the selective isolation and presumptive identification of pseudomonas aeruginosa the medium was first developed by lowburry and is a modification of tech agar developed by king et al. Lowbury and collins 6 studied cetrimide as a selective agent. Pseudomonas cepacia cfc agar, or pseudomonas aeruginosa cn agar. Pseudomonas species also typically give a positive result to the oxidase test, the absence of gas formation from glucose, glucose is oxidised in oxidationfermentation test using hugh and leifson of test, beta hemolytic on blood agar, indole negative, methyl red negative, vogesproskauer test negative, and citrate positive. These are not pure cultures and a variety of colony morphologies are visible.